Effects of mediator antagonism on mannitol and adenosine monophosphate challenges

Clin Exp Allergy. 2003 Jun;33(6):783-8. doi: 10.1046/j.1365-2222.2003.01688.x.

Abstract

Background: Airway hyper-responsiveness (AHR) to indirect stimuli is a useful non-invasive surrogate inflammatory marker in the evaluation of asthma, while histamine and cysteinyl leukotrienes are important inflammatory mediators.

Objective: To evaluate AHR to indirect bronchoconstrictor stimuli and time taken to recover following single doses of montelukast 10 mg and desloratadine 5 mg in combination, montelukast 10 mg alone and placebo.

Methods: Fifteen mild-to-moderate persistent asthmatics completed a randomized, double-blind, cross-over study. Patients received encapsulated montelukast 10 mg/desloratadine 5 mg combination, montelukast 10 mg alone and placebo, 10-14 h prior to challenge on two separate occasions. The mannitol threshold dose, AMP threshold concentration and recovery times after challenge were measured along with lung function.

Results: Compared to placebo, montelukast/desloratadine conferred improvements (P < 0.05) in adenosine monophosphate (AMP) threshold concentration and mannitol threshold dose: a 3.2-fold (95% CI 2.2-4.6) and 2.4-fold (95% CI 1.7-3.3) difference, respectively, while compared to montelukast this amounted to a 2.0-fold (95% CI 1.2-3.4) and 1.5-fold (95% CI 1.1-2.4) improvement, respectively. Montelukast was not significantly different from placebo. Both montelukast/desloratadine and montelukast compared to placebo, shortened recovery following both challenges (P < 0.05): a 27-min (95% CI 17-37) and 29-min (95% CI 20-36) reduction, respectively, for AMP, and a 27-min (95% CI 17-37) and 26-min (95% CI 17-35) reduction, respectively for mannitol.

Conclusion: The dissociated effects of single doses of montelukast alone but not montelukast/desloratadine combination on AHR and recovery time, highlights the relative roles of histamine in initiating the bronchoconstrictor response and cysteinyl leukotrienes in sustaining it. Similar improvements in AHR and recovery time were observed following both indirect bronchoconstrictor stimuli.

Publication types

  • Clinical Trial
  • Comparative Study
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates*
  • Adenosine Monophosphate
  • Adult
  • Asthma / immunology*
  • Asthma / physiopathology
  • Bronchial Hyperreactivity
  • Bronchial Provocation Tests / methods
  • Bronchoconstrictor Agents
  • Cross-Over Studies
  • Cyclopropanes
  • Double-Blind Method
  • Female
  • Histamine H1 Antagonists*
  • Humans
  • Leukotriene Antagonists*
  • Loratadine* / analogs & derivatives
  • Male
  • Mannitol
  • Middle Aged
  • Quinolines*
  • Sulfides
  • Time Factors

Substances

  • Acetates
  • Bronchoconstrictor Agents
  • Cyclopropanes
  • Histamine H1 Antagonists
  • Leukotriene Antagonists
  • Quinolines
  • Sulfides
  • Mannitol
  • Adenosine Monophosphate
  • Loratadine
  • desloratadine
  • montelukast