Four new cycloartane glycosides from Aquilegia vulgaris and their immunosuppressive activities in mouse allogeneic mixed lymphocyte reaction

Makiko Nishida; Hitoshi Yoshimitsu; Masafumi Okawa; Toshihiro Nohara

doi:10.1248/cpb.51.683

Four new cycloartane glycosides from Aquilegia vulgaris and their immunosuppressive activities in mouse allogeneic mixed lymphocyte reaction

Chem Pharm Bull (Tokyo). 2003 Jun;51(6):683-7. doi: 10.1248/cpb.51.683.

Authors

Makiko Nishida¹, Hitoshi Yoshimitsu, Masafumi Okawa, Toshihiro Nohara

Affiliation

¹ Faculty of Engineering, Kyushu Kyoritsu University, Kitakyushu, Japan.

PMID: 12808247
DOI: 10.1248/cpb.51.683

Abstract

Four new cycloartane glycosides, named aquilegiosides C-F, were isolated from the dried aerial parts of Aquilegia vulgaris. Their structures were determined by two dimensional (2D) NMR spectroscopic analysis and chemical evidence. Aquilegiosides C-F suppressed the proliferation of lymphocytes in mouse allogeneic mixed lymphocyte reaction with IC(50), ranging from 3.7x10(-5) to 2.2x10(-4) M.

MeSH terms

Animals
Aquilegia / chemistry*
Cell Division / drug effects
Cell Division / immunology
Glycosides / chemistry
Glycosides / isolation & purification*
Glycosides / pharmacology
Immunosuppressive Agents / chemistry
Immunosuppressive Agents / isolation & purification*
Immunosuppressive Agents / pharmacology
In Vitro Techniques
Inhibitory Concentration 50
Lymphocytes / drug effects*
Lymphocytes / immunology
Magnetic Resonance Spectroscopy
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Molecular Structure
Plant Components, Aerial / chemistry
Plant Extracts / chemistry
Plant Extracts / isolation & purification
Plant Extracts / pharmacology
Triterpenes / chemistry*

Substances

Glycosides
Immunosuppressive Agents
Plant Extracts
Triterpenes
cycloartane