Synthesis of HyBeacons and dual-labelled probes containing 2'-fluorescent groups for use in genetic analysis

Neil Dobson; David G McDowell; David J French; Lynda J Brown; John M Mellor; Tom Brown

doi:10.1039/b302855k

Synthesis of HyBeacons and dual-labelled probes containing 2'-fluorescent groups for use in genetic analysis

Chem Commun (Camb). 2003 Jun 7:(11):1234-5. doi: 10.1039/b302855k.

Authors

Neil Dobson¹, David G McDowell, David J French, Lynda J Brown, John M Mellor, Tom Brown

Affiliation

¹ Department of Chemistry, University of Southampton, Highfield, Southampton, UK SO17 1BJ.

PMID: 12809207
DOI: 10.1039/b302855k

Abstract

An FMOC-protected 2'-hydroxyethyl uridine phosphoramidite has been used to synthesise fluorescein-labelled HyBeacon probes and "FAM-ROX" dual-labelled fluorogenic oligonucleotides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Energy Transfer
Fluorescence
Fluorescent Dyes / chemical synthesis*
Sequence Analysis, DNA*

Substances

Fluorescent Dyes