Genome analysis of a novel Shiga toxin 1 (Stx1)-converting phage which is closely related to Stx2-converting phages but not to other Stx1-converting phages

J Bacteriol. 2003 Jul;185(13):3966-71. doi: 10.1128/JB.185.13.3966-3971.2003.

Abstract

Two Stx-converting phages, designated Stx1 phi and Stx2 phi-II, were isolated from an Escherichia coli O157:H7 strain, Morioka V526, and their entire nucleotide sequences were determined. The genomes of both phages were similar except for the stx gene-flanking regions. Comparing these phages to other known Stx-converting phages, we concluded that Stx1 phi is a novel Stx1-converting phage closely related to Stx2-converting phages so far reported.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coliphages / genetics
  • Coliphages / isolation & purification*
  • Coliphages / metabolism
  • Escherichia coli O157 / virology*
  • Genome, Viral*
  • Humans
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Sequence Analysis, DNA*
  • Shiga Toxin 1 / genetics
  • Shiga Toxin 1 / metabolism*
  • Shiga Toxin 2 / genetics
  • Shiga Toxin 2 / metabolism*
  • Viral Proteins / genetics

Substances

  • Shiga Toxin 1
  • Shiga Toxin 2
  • Viral Proteins

Associated data

  • GENBANK/AP005153
  • GENBANK/AP005154