Abstract
A putative isocitrate dehydrogenase (IDH) cDNA from alfalfa has been cloned and sequenced. The derived amino acid sequence of 433 residues contains the isocitrate and isopropylmalate dehydrogenase signatures, is 63% identical to yeast mitochondrial NADP-IDH and shares high sequence identity with peptides of pig heart NADP-IDH. The sequence contains a potential N-terminal leader with similarities to a thylakoid transit peptide. IDH transcripts and NADP-IDH activity were detected in all alfalfa tissues examined, their levels depending upon the tissue type and its developmental stage. Transcripts and enzymatic activity were not induced on exposure of cell suspension cultures to a fungal elicitor. IDH is encoded by a small gene family in alfalfa.
MeSH terms
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3-Isopropylmalate Dehydrogenase
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Alcohol Oxidoreductases / genetics
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Blotting, Southern
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Cloning, Molecular
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DNA / genetics
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DNA / isolation & purification
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Isocitrate Dehydrogenase / genetics*
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Isocitrate Dehydrogenase / metabolism
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Medicago sativa / enzymology*
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Medicago sativa / genetics
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Mitochondria / enzymology
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Mitochondria, Heart / enzymology
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Molecular Sequence Data
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Molecular Weight
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Open Reading Frames
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RNA / genetics
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RNA / isolation & purification
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Recombinant Proteins / metabolism
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics
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Sequence Homology, Amino Acid
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Swine
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Transcription, Genetic
Substances
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Recombinant Proteins
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RNA
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DNA
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Alcohol Oxidoreductases
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Isocitrate Dehydrogenase
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3-Isopropylmalate Dehydrogenase