Inhibition of repair of bleomycin-induced DNA strand breaks by 2'-deoxycoformycin and its effect on antitumor activity in L5178Y lymphoblasts

Biochem Pharmacol. 1992 Dec 1;44(11):2229-33. doi: 10.1016/0006-2952(92)90351-i.

Abstract

We have observed previously that treatment of plateau-phase L5178Y murine lymphoblasts in vitro with 2'-deoxycoformycin plus deoxyadenosine (dCF/dAdo) can inhibit the repair of X-irradiation-induced DNA single-strand breaks (SSB) in these cells and that this effect is associated with synergistic cell kill. In this study we examined the effect of a combination treatment of plateau-phase L5178Y cells with bleomycin (BLM) plus dCF/dAdo. Incubation of BLM-treated cells with dCF/dAdo resulted in significant inhibition of the repair of BLM-induced DNA SSB. However, an additive, but not a synergistic, increase in cell kill was observed when cells were treated with a combination of BLM plus dCF/dAdo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Combined Chemotherapy Protocols / pharmacology
  • Bleomycin / toxicity*
  • DNA Damage*
  • DNA Repair / drug effects*
  • DNA, Neoplasm / drug effects*
  • DNA, Neoplasm / physiology
  • DNA, Single-Stranded / drug effects
  • Deoxyadenosines / pharmacology
  • Drug Synergism
  • Leukemia L5178 / drug therapy*
  • Leukemia L5178 / pathology
  • Leukemia L5178 / physiopathology*
  • Mice
  • Mice, Inbred DBA
  • Pentostatin / pharmacology*

Substances

  • DNA, Neoplasm
  • DNA, Single-Stranded
  • Deoxyadenosines
  • Bleomycin
  • Pentostatin