Combined chemotherapy including high-dose methotrexate in KSHV/HHV8-associated primary effusion lymphoma

Am J Hematol. 2003 Jul;73(3):143-8. doi: 10.1002/ajh.10341.

Abstract

Primary effusion lymphoma (PEL) is a rare KSHV/HHV8-associated high-grade non-Hodgkin's lymphoma (NHL) of B-cell origin, characterized by serous effusions in body cavities. Most patients are HIV-infected homosexual men with severe immunosuppression and other KSHV/HHV8-associated diseases such as Kaposi's sarcoma (KS). The prognosis is poor with a median survival of less than 6 months in most cohorts. The achievement of a sustained complete remission is rare. High-dose chemotherapy regimens are warranted to improve complete remission rate and survival. Seven patients with AIDS-associated PEL were treated with a combined chemotherapy including high-dose methotrexate followed by leucovorin rescue. In all cases, KSHV/HHV8 sequences were detected in the effusion samples using quantitative PCR assays. Five patients had a pre-existing KS, associated in three cases with multicentric Castleman's disease (MCD). Upon diagnosis, 6 patients received antiretroviral therapy, which was maintained during chemotherapy in 5 of them. At time of analysis, 3 out of 7 patients were in complete remission 18, 26, and 78 months after PEL diagnosis. Three patients died with a progressive PEL at 22, 67, and 153 days after diagnosis, and 1 patient died 9 months after PEL diagnosis with a MCD-associated plasmablastic NHL. Complete remission was obtained in 3 out of 7 patients treated for AIDS-associated PEL with combined chemotherapy containing high-dose methotrexate.

MeSH terms

  • Acquired Immunodeficiency Syndrome / complications*
  • Acquired Immunodeficiency Syndrome / drug therapy
  • Adult
  • Antigens, CD / blood
  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use*
  • Antiretroviral Therapy, Highly Active*
  • DNA, Viral / isolation & purification
  • France
  • HIV Infections / complications
  • HIV Infections / drug therapy
  • Herpesviridae Infections / blood
  • Herpesviridae Infections / drug therapy*
  • Herpesviridae Infections / immunology
  • Herpesvirus 8, Human* / isolation & purification
  • Homosexuality, Male
  • Humans
  • Immunophenotyping
  • Male
  • Methotrexate / therapeutic use*
  • Middle Aged
  • Pleural Effusion / pathology
  • Prognosis
  • RNA, Viral / blood
  • Sarcoma, Kaposi / blood
  • Sarcoma, Kaposi / drug therapy*
  • Sarcoma, Kaposi / immunology
  • Sarcoma, Kaposi / mortality
  • Sarcoma, Kaposi / pathology
  • Treatment Outcome
  • Viral Load
  • White People

Substances

  • Antigens, CD
  • DNA, Viral
  • RNA, Viral
  • Methotrexate