Abstract
Egg white lysozyme was found to catalyze the transfer of N-acetylglucosamine to cyclo[-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->] (CTS). Structural analysis showed that the transfer product was 3-O-beta-N-acetylglucosaminyl CTS, cyclo[-->6)-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->6)-[beta-GlcNAc-(1-->3)]-alpha-D-Glcp-(1-->3)-alpha-D-Glcp-(1-->]. This branched saccharide is anticipated to be a model compound of the sugar chains of glycoproteins.
MeSH terms
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Animals
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Carbohydrate Sequence
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Catalysis
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Chick Embryo
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Chromatography, Affinity
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Chromatography, High Pressure Liquid
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Egg White
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Glycosylation
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Indicators and Reagents
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Magnetic Resonance Spectroscopy
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Molecular Sequence Data
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Molecular Weight
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Muramidase / chemistry*
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Oligosaccharides / chemical synthesis*
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Oligosaccharides / chemistry
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Spectrometry, Mass, Fast Atom Bombardment
Substances
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Indicators and Reagents
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Oligosaccharides
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cyclo(-6)-glucopyranosyl-1-3-glucopyranosyl-1-6-(N-acetylglucosaminyl-1-3)glucopyranosyl-1-3-glucopyranosyl-(1-)
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Muramidase