Quantitation of DNA damage by an aldehyde reactive probe (ARP)

S Kurisu; T Miya; H Terato; A Masaoka; Y Ohyama; K Kubo; H Ide

doi:10.1093/nass/1.1.45

Quantitation of DNA damage by an aldehyde reactive probe (ARP)

Nucleic Acids Res Suppl. 2001:(1):45-6. doi: 10.1093/nass/1.1.45.

Authors

S Kurisu¹, T Miya, H Terato, A Masaoka, Y Ohyama, K Kubo, H Ide

Affiliation

¹ Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.

PMID: 12836256
DOI: 10.1093/nass/1.1.45

Abstract

DNA is constantly damaged by endogenous and environmental agents. Abasic sites representing a major class of DNA damage can be quantitated by an ELISA-like assay using an aldehyde reactive probe (ARP). It is shown that oxidative pyrimidine lesions can be also quantitated by the ARP assay in combination with the treatment with endonuclease III.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Aldehydes / analysis*
Aldehydes / chemistry
Biotinylation
DNA / chemistry
DNA Damage*
Deoxyribonuclease (Pyrimidine Dimer)*
Endodeoxyribonucleases
Enzyme-Linked Immunosorbent Assay
Escherichia coli Proteins*
HeLa Cells
Humans
Luminescent Measurements
Molecular Probes / chemistry*

Substances

Aldehydes
Escherichia coli Proteins
Molecular Probes
DNA
Endodeoxyribonucleases
Deoxyribonuclease (Pyrimidine Dimer)
NTH protein, E coli