Molecular design of hairpin imidazole-pyrrole polyamide for effective DNA alkylation

T Bando; A Narita; I Saito; H Sugiyama

doi:10.1093/nass/1.1.225

Molecular design of hairpin imidazole-pyrrole polyamide for effective DNA alkylation

Nucleic Acids Res Suppl. 2001:(1):225-6. doi: 10.1093/nass/1.1.225.

Authors

T Bando¹, A Narita, I Saito, H Sugiyama

Affiliation

¹ CREST, Japan Science and Technology Corporation (JST), Japan.

PMID: 12836346
DOI: 10.1093/nass/1.1.225

Abstract

A new type of hairpin polyamide-CPI conjugate possessing a vinyl linker has been synthesized. Sequence-selective alkylation of double-stranded DNA by conjugates was investigated by high resolution denaturing gel electrophoresis using 450 bp DNA fragments. Highly-efficient DNA alkylation predominantly occurs at the purines of 5'-(A/T)G(T/A)CPu-3' site at nanomolar concentration.

MeSH terms

Alkylation
DNA / chemistry*
Imidazoles / chemistry*
Nucleic Acid Conformation
Nylons / chemical synthesis*
Nylons / chemistry*
Purines / chemistry
Pyrroles / chemistry*

Substances

Imidazoles
Nylons
Purines
Pyrroles
DNA
N-methylpyrrole
1-methylimidazole