Bisulfite-based methylation analysis of imprinted genes

Methods Mol Biol. 2001:181:217-28. doi: 10.1385/1-59259-211-2:217.

Abstract

Genomic imprinting is an epigenetically controlled form of gene regulation leading to the preferential expression of one parental gene copy. To date, approximately 40 imprinted genes have been described that are exclusively or predominantly expressed from either the paternal or the maternal allele (www.mgu.har.mrc.ac.uk/imprinting/implink.html). Changes in the imprinted expression of such genes result in developmental abnormalities; in the human they are associated with several diseases and various types of cancer (1-3).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA Methylation*
  • Genetic Techniques*
  • Genomic Imprinting / genetics*
  • Humans
  • Molecular Structure
  • Polymerase Chain Reaction
  • Sulfites / chemistry*

Substances

  • Sulfites
  • hydrogen sulfite