Molecular mechanism of agonism and inverse agonism in the melanocortin receptors: Zn(2+) as a structural and functional probe

Ann N Y Acad Sci. 2003 Jun:994:1-11. doi: 10.1111/j.1749-6632.2003.tb03156.x.

Abstract

Among the rhodopsin-like 7TM receptors, the MC receptors are functionally unique because their high constitutive signaling activity is regulated not only by endogenous peptide agonists-MSH peptides-but also by endogenous inverse agonists, namely, the proteins agouti and AGRP. Moreover, the metal-ion Zn(2+) increases the signaling activity of at least the MC1 and MC4 receptors in three distinct ways: (1). by directly functioning as an agonist; (2). by potentiating the action of the endogenous agonist; and (3). by inhibiting the binding of the endogenous inverse agonist. Structurally the MC receptors are part of a small subset of 7TM receptors in which the main ligand-binding crevice, and especially extracellular loops 2 and 3, appear to be specially designed for easy ligand access and bias towards an active state of the receptor-i.e., constitutive activity. Thus, in the MC receptors extracellular loop 2 is ultrashort because TM-IV basically connects directly into TM-V, whereas extracellular loop 3 appears to be held in a particular, constrained conformation by a putative, internal disulfide bridge. The interaction mode for the small and well-defined zinc-ion between a third, free Cys residue in extracellular loop 3 and conceivably an Asp residue located at the inner face of TM-III gives important information concerning the activation mechanism for the MC receptors.

Publication types

  • Review

MeSH terms

  • Animals
  • Binding Sites
  • Humans
  • Ligands
  • Melanocyte-Stimulating Hormones / chemistry
  • Melanocyte-Stimulating Hormones / metabolism*
  • Models, Molecular
  • Molecular Structure
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Receptors, Corticotropin / agonists*
  • Receptors, Corticotropin / chemistry
  • Receptors, Corticotropin / genetics
  • Receptors, Corticotropin / metabolism
  • Receptors, Melanocortin
  • Rhodopsin / chemistry
  • Signal Transduction / physiology*
  • Zinc / metabolism*

Substances

  • Ligands
  • Receptors, Corticotropin
  • Receptors, Melanocortin
  • Melanocyte-Stimulating Hormones
  • Rhodopsin
  • Zinc