Induction of apoptosis and G2/M cell cycle arrest by Gleditsioside E from Gleditsia sinensis in HL-60 cells

Planta Med. 2003 Jun;69(6):561-3. doi: 10.1055/s-2003-40645.

Abstract

Gleditsioside E, a triterpene saponin isolated from Gleditsia sinensis, showed significant cytotoxicity against Bel-7402, BGC-823, HeLa, HL-60 and MCF-7 cell lines. The results of flow cytometry with annexin V-FITC/PI double staining proved that gleditsioside E mainly induced early apoptosis in HL-60 cells. Gleditsioside E treatment resulted in a prominent increase of the G 2 /M population in HL-60 cells, and an accumulation of the sub-G 1 (hypoploid) peak was observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Apoptosis / drug effects
  • Cell Cycle / drug effects*
  • Flow Cytometry
  • Fruit
  • Gleditsia*
  • HL-60 Cells / drug effects
  • Humans
  • Inhibitory Concentration 50
  • Paclitaxel / pharmacology
  • Phytotherapy*
  • Plant Extracts / pharmacology*
  • Saponins / pharmacology*
  • Tumor Cells, Cultured / drug effects

Substances

  • Antineoplastic Agents, Phytogenic
  • Plant Extracts
  • Saponins
  • gleditsioside E
  • Paclitaxel