Abstract
Bioassay-guided fractionation of the BuOH-soluble extract of Fritillaria ussuriensis afforded verticinone ( 1), verticine ( 2), and peimisine ( 3). Purification of these compounds was achieved with the use of various chromatographic methods. The structures of the compounds were identified on the basis of MS and NMR data analysis. Compounds 1 - 3 inhibited angiotensin I converting enzyme activity in a dose-dependent manner, displaying 50 % inhibitory concentration values of 165.0 microM, 312.8 microM, 526.5 microM, respectively. The presence of these active substances may be responsible, at least in part, for the antihypertensive action of the bulbs of Fritillaria ussuriensis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaloids / administration & dosage
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Alkaloids / pharmacology*
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Alkaloids / therapeutic use
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Angiotensin-Converting Enzyme Inhibitors / administration & dosage
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Angiotensin-Converting Enzyme Inhibitors / pharmacology*
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Angiotensin-Converting Enzyme Inhibitors / therapeutic use
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Cevanes / pharmacology
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Dose-Response Relationship, Drug
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Fritillaria*
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Humans
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Inhibitory Concentration 50
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Peptidyl-Dipeptidase A / drug effects
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Phytotherapy*
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Plant Extracts / administration & dosage
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Plant Extracts / pharmacology*
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Plant Extracts / therapeutic use
Substances
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Alkaloids
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Angiotensin-Converting Enzyme Inhibitors
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Cevanes
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Plant Extracts
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peimisine
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verticine
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Peptidyl-Dipeptidase A