Single-scattering spectroscopy for the endoscopic analysis of particle size in superficial layers of turbid media

Appl Opt. 2003 Jul 1;42(19):4095-101. doi: 10.1364/ao.42.004095.

Abstract

We report on the development of an optical-fiber-based diagnostic tool that is sensitive to single-scattering events close to the fiber-optic probe tip. By using a single fiber to deliver and detect white light we optimised the detection probability of singly scattered photons from small depths. The sampling depth of this delivery-and-collection fiber was investigated by use of a tissue phantom. We found that for our phantom 90% of the single-scattering signal in the delivery-and-collection fiber originated from less than 200 microm from the fiber tip. The contribution of multiply scattered light from a greater depth to the signal was measured with an additional collection fiber. Several tissue phantoms demonstrated our fiber-optic probes sensitivity to light scattering from superficial layers of tissue and thereby its potential to detect superficial precancerous epithelial lesions.

Publication types

  • Evaluation Study

MeSH terms

  • Anisotropy
  • Computer-Aided Design
  • Endoscopy / methods*
  • Epithelium / physiology
  • Fiber Optic Technology / instrumentation*
  • Fiber Optic Technology / methods*
  • Nephelometry and Turbidimetry / instrumentation*
  • Nephelometry and Turbidimetry / methods*
  • Optical Fibers
  • Particle Size
  • Phantoms, Imaging
  • Reproducibility of Results
  • Scattering, Radiation
  • Sensitivity and Specificity
  • Spectrophotometry / instrumentation*
  • Spectrophotometry / methods*
  • Surface Properties