Abstract
HPLC was used to analyze doxorubicin in multidrug-resistance (MDR) human carcinoma cells. This method is novel, simple, sensitive, linear, accurate and precise. The minimal detectable concentration is 0.2 microg ml(-1). The reversal effects of tea polyphenols on MDR are elucidated by this method. The results indicate that the tea polyphenol, (-)-epigallocatechin gallate, is a potential modulator of MDR.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Carcinoma, Squamous Cell / metabolism*
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Carcinoma, Squamous Cell / pathology
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Carcinoma, Squamous Cell / physiopathology
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Catechin / analogs & derivatives*
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Catechin / pharmacology*
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Cell Division / drug effects
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Cell Survival / drug effects
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Chromatography, High Pressure Liquid / methods
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Doxorubicin / analysis*
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Doxorubicin / pharmacology*
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Drug Resistance, Multiple*
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Flavonoids*
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Humans
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Mouth Neoplasms / metabolism
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Mouth Neoplasms / physiopathology
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Phenols / pharmacology*
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Polymers / pharmacology*
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Tea / chemistry
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Tumor Cells, Cultured
Substances
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Flavonoids
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Phenols
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Polymers
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Tea
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Doxorubicin
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Catechin
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epigallocatechin gallate