Microbial transformation of ginsenoside Rb1 by Rhizopus stolonifer and Curvularia lunata

Biotechnol Lett. 2003 Feb;25(4):339-44. doi: 10.1023/a:1022320824000.

Abstract

Of 49 microbial strains screened for their capabilities to transform ginsenoside Rb1, Rhizopus stolonifer and Curvularia lunata produced four key metabolites: 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl]-3beta,12beta, 20(S)-trihydroxydammar-24-ene (1), 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl]-3beta,12beta, 20(S)-trihydroxydammar-24-ol (2), 3-O-[beta-D-glucopyranosyl-(1,2)-beta-D-glucopyranosyl]-3beta, 12beta, 20(S)-trihydroxydammar-24-ene (3), and 3-O-beta-D-glucopyranosyl-3beta, 12beta, 20(S)-trihydroxydammar-24-ene (4), identified by TOF-MS, 1H- and 13C-NMR spectral data. Metabolites 1, 3 and 4 were from the incubation with R. stolonifer, and 1 and 2 from the incubation with C. lunata. Compound 2 was identified as a new compound.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascomycota / chemistry
  • Ascomycota / classification
  • Ascomycota / metabolism*
  • Biotransformation
  • Chromatography, Thin Layer / methods
  • Ginsenosides / chemistry*
  • Ginsenosides / metabolism*
  • Rhizopus / chemistry
  • Rhizopus / classification
  • Rhizopus / metabolism*
  • Species Specificity

Substances

  • Ginsenosides
  • ginsenoside Rb1