Abstract
Inducible costimulator (ICOS) is a novel costimulatory molecule expressed in activated T cell and has critical regulation effect on special immune response. In this study, the cDNA encoding human ICOS was cloned from activated tonsil cells via RT-PCR, and was expressed in E. coli on pET28 expression vector. The recombinant ICOS protein expressed from E. coli showed a molecular weight of 14 kD on SDS-polyacrylamide gel electrophoresis and was further confirmed by Western blot. In presence of IL-10, the purified rhICOS significantly increased in vitro B cell growth stimulated by pokeweed mitogen (PWM), and enhanced the secretion of IgG from B cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Differentiation, T-Lymphocyte / biosynthesis*
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Antigens, Differentiation, T-Lymphocyte / isolation & purification
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Antigens, Differentiation, T-Lymphocyte / pharmacology
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B-Lymphocytes / drug effects*
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Cloning, Molecular
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Escherichia coli / genetics
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Genetic Vectors
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Humans
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Immunoglobulin G / biosynthesis
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Inducible T-Cell Co-Stimulator Protein
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Lymphocyte Activation / drug effects
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Recombinant Proteins / biosynthesis*
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / pharmacology
Substances
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Antigens, Differentiation, T-Lymphocyte
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ICOS protein, human
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Immunoglobulin G
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Inducible T-Cell Co-Stimulator Protein
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Recombinant Proteins