Abstract
Reelin is an extracellular protein that is crucial for layer formation in the embryonic brain. Here, we demonstrate that Reelin functions postnatally to regulate the development of the neuromuscular junction. Reelin is required for motor end-plate maturation and proper nerve-muscle connectivity, and it directly promotes synapse elimination. Unlike layer formation, neuromuscular junction development requires a function of Reelin that is not mediated by Disabled1 or very-low-density lipoprotein receptors and apolipoprotein E receptor 2 receptors but by a distinct mechanism involving its protease activity.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
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Retracted Publication
MeSH terms
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Action Potentials
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Animals
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Axons / metabolism
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Cell Adhesion Molecules, Neuronal / genetics
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Cell Adhesion Molecules, Neuronal / metabolism
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Cell Adhesion Molecules, Neuronal / pharmacology
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Cell Adhesion Molecules, Neuronal / physiology*
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Culture Media, Conditioned
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Diaphragm / innervation
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Extracellular Matrix Proteins / genetics
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Extracellular Matrix Proteins / metabolism
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Extracellular Matrix Proteins / pharmacology
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Extracellular Matrix Proteins / physiology*
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LDL-Receptor Related Proteins
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Mice
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Mice, Neurologic Mutants
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Microscopy, Confocal
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Microscopy, Electron
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Motor Endplate / ultrastructure
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Motor Neurons / metabolism
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Muscle, Skeletal / innervation
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Mutation
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Neuromuscular Junction / growth & development*
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Neuromuscular Junction / metabolism
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Neuromuscular Junction / physiology*
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Neuromuscular Junction / ultrastructure
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Receptors, LDL / genetics
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Receptors, LDL / metabolism
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Receptors, Lipoprotein / genetics
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Receptors, Lipoprotein / metabolism
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Reelin Protein
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Schwann Cells / metabolism
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Serine Endopeptidases
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Serine Proteinase Inhibitors / pharmacology
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Sulfones / pharmacology
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Synapses / physiology*
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Synapses / ultrastructure
Substances
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Cell Adhesion Molecules, Neuronal
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Culture Media, Conditioned
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Dab1 protein, mouse
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Extracellular Matrix Proteins
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LDL-Receptor Related Proteins
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Nerve Tissue Proteins
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Receptors, LDL
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Receptors, Lipoprotein
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Reelin Protein
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Serine Proteinase Inhibitors
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Sulfones
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VLDL receptor
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low density lipoprotein receptor-related protein 8
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4-(2-aminoethyl)benzenesulfonylfluoride
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Reln protein, mouse
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Serine Endopeptidases