Abstract
The cell wall of the environmental pathogen Mycobacterium avium is important to its virulence and intrinsic antimicrobial resistance. To identify genes involved in cell wall biosynthesis, "transposome" insertion libraries were screened for mutants with altered colony morphology on medium containing the lipoprotein stain Congo red. Nineteen such mutants were isolated and mapped, including 10 with insertions in a functional island of cell wall biosynthetic genes that spans approximately 40 kb of the M. avium genome.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Cell Wall / genetics
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Cell Wall / metabolism*
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Congo Red / metabolism
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DNA Transposable Elements
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Gene Library
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Glycolipids / metabolism
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Glycopeptides / metabolism
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Mutagenesis, Insertional
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Mutation
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Mycobacterium avium / genetics*
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Mycobacterium avium / growth & development
Substances
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Bacterial Proteins
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DNA Transposable Elements
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Glycolipids
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Glycopeptides
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Congo Red