Synthesis and properties of 5'-amino-2',4'-BNA modified oligonucleotides with P3'-->N5' phosphoramidate linkages

Satoshi Obika; Osamu Nakagawa; Akiko Hiroto; Yoshiyuki Hari; Takeshi Imanishi

doi:10.1093/nass/2.1.25

Synthesis and properties of 5'-amino-2',4'-BNA modified oligonucleotides with P3'-->N5' phosphoramidate linkages

Nucleic Acids Res Suppl. 2002:(2):25-6. doi: 10.1093/nass/2.1.25.

Authors

Satoshi Obika¹, Osamu Nakagawa, Akiko Hiroto, Yoshiyuki Hari, Takeshi Imanishi

Affiliation

¹ Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.

PMID: 12903087
DOI: 10.1093/nass/2.1.25

Abstract

5'-Amino-2',4'-BNA, a novel analogue of BNA series compounds, was synthesized and its incorporation into oligonucleosides via P3'-->N5' phosphoramidate linkage was successfully achieved on a DNA automatic synthesizer. The 5'-amino-2',4'-BNA modified oligonucleotides were found to have a potent hybridizing affinity for the single stranded RNA and double stranded DNA complements, and also to show a good resistant ability against SVPDE.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amides / chemistry*
Base Sequence
Nucleic Acids / chemical synthesis*
Nucleic Acids / chemistry
Oligonucleotides / chemistry
Phosphoric Acids / chemistry*

Substances

Amides
Nucleic Acids
Oligonucleotides
Phosphoric Acids
phosphoramidic acid