2-Amino-6-vinyl purine as a tool for post synthetic conjugation of DNA with radio-, spin-, fluorescence labels and peptides

Nucleic Acids Res Suppl. 2002:(2):149-50. doi: 10.1093/nass/2.1.149.

Abstract

We have previously demonstrated that 2-amino-6-vinylpurine is an efficient cross-linking agent with high selectivity towards cytidine. In this study, we applied this nucleobase analog to versatile conjugation of oligodeoxynucleotides with radio-, spin-, fluorescence- and peptide labels. The oligodeoxynucleotides incorporating 2-amino-6-vinylpurine nucleoside were reacted with amino- bearing nucleophies in an excess amount to give the corresponding conjugates in good yield.

MeSH terms

  • Base Sequence
  • Chromatography, High Pressure Liquid
  • DNA / chemistry*
  • Fluorescent Dyes / chemistry*
  • Peptides / chemistry*
  • Purines / chemistry*
  • Radioisotopes / chemistry*
  • Spin Labels*
  • Vinyl Compounds / chemistry*

Substances

  • 2-amino-6-vinylpurine
  • Fluorescent Dyes
  • Peptides
  • Purines
  • Radioisotopes
  • Spin Labels
  • Vinyl Compounds
  • DNA