Abstract
The dengue virus molecular typing method described by Lanciotti and coworkers (R. S. Lanciotti, C. H. Calisher, D. J. Gubler, G. J. Chang, and A. Vance-Vorndam, J. Clin. Microbiol. 30:545-551, 1992) is used worldwide for diagnosis and surveillance. However, it failed to detect DENV-1 variants in Cambodia due to a point mutation. We describe an improvement of the method that allows the detection of additional DENV-1 strains, including potential variants.
MeSH terms
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Base Sequence
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Dengue / diagnosis
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Dengue / prevention & control
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Dengue Virus / classification
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Dengue Virus / genetics*
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Dengue Virus / isolation & purification*
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Gene Amplification
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Genes, Viral
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Geography
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Humans
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Molecular Sequence Data
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Polymerase Chain Reaction / methods
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Reverse Transcriptase Polymerase Chain Reaction
Associated data
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GENBANK/A75711
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GENBANK/AB074760
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GENBANK/AB074761
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GENBANK/AF180817
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GENBANK/AF180818
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GENBANK/AF226686
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GENBANK/AF226687
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GENBANK/AF298807
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GENBANK/AF298808
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GENBANK/AF309641
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GENBANK/AF350498
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GENBANK/AF459605
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GENBANK/AF459606
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GENBANK/AF459607
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GENBANK/AF459608
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GENBANK/AF538024
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GENBANK/AF538027
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GENBANK/AF538028
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GENBANK/AF538031
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GENBANK/AX224209
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GENBANK/AX224211
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GENBANK/AX224213
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GENBANK/AX224215
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GENBANK/AX224235
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GENBANK/AY079173
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GENBANK/D00501
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GENBANK/D00502
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GENBANK/D00503
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GENBANK/E06832
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GENBANK/M23027
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GENBANK/M87512
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GENBANK/S64849
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GENBANK/S75335
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GENBANK/U88535
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GENBANK/U88536
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GENBANK/U88537
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GENBANK/Z74047