Abstract
Virus-like particles of Dijon171/96 virus, a genogroup II norovirus, were expressed in a baculovirus system and were used for a seroepidemiological study of 1078 age-stratified human sera collected in Dijon, France. The results showed a seroprevalence of 74.1%. Furthermore, we showed that murine antibodies generated against recombinant Dijon171/96 virus, and human antibodies recognized discontinuous epitopes on the particles.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aged
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Animals
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Antibodies, Viral / blood
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Caliciviridae / genetics*
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Caliciviridae / immunology
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Caliciviridae / isolation & purification*
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Caliciviridae Infections / epidemiology*
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Caliciviridae Infections / immunology
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Capsid Proteins / genetics
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Capsid Proteins / isolation & purification
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Child
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Child, Preschool
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Cloning, Molecular / methods
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Electrophoresis, Polyacrylamide Gel
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Enzyme-Linked Immunosorbent Assay
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France / epidemiology
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Humans
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Immunoglobulin G / blood
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Infant
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Mice
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Middle Aged
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Molecular Sequence Data
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Seroepidemiologic Studies
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Serotyping / methods
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Substances
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Antibodies, Viral
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Capsid Proteins
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Immunoglobulin G