Diagnosis of dehydratase inhibitors in melanin biosynthesis inhibitor (MBI-D) resistance by primer-introduced restriction enzyme analysis in scytalone dehydratase gene of Magnaporthe grisea

Pest Manag Sci. 2003 Aug;59(8):843-6. doi: 10.1002/ps.742.

Abstract

We have established a simple diagnosis method for rice blast fungus resistant to MBI-D. This involves the preparation of PCR templates directly from the lesions in combination with primer-introduced restriction enzyme analysis PCR (PIRA-PCR).

MeSH terms

  • Amides / pharmacology
  • Base Sequence
  • Cyclopropanes / pharmacology
  • DNA Mutational Analysis / methods
  • DNA Primers / genetics*
  • Drug Resistance, Fungal / genetics*
  • Genes, Fungal / genetics*
  • Hydro-Lyases / antagonists & inhibitors*
  • Hydro-Lyases / genetics*
  • Magnaporthe / drug effects*
  • Magnaporthe / enzymology
  • Magnaporthe / genetics*
  • Melanins / biosynthesis
  • Oryza / microbiology
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Restriction Mapping

Substances

  • Amides
  • Cyclopropanes
  • DNA Primers
  • Melanins
  • Hydro-Lyases
  • scytalone dehydratase
  • carpropamid