Diagnosis of hepatocellular carcinoma by fine needle aspiration cytology. Cellular features

Acta Cytol. 2003 Jul-Aug;47(4):581-9. doi: 10.1159/000326572.

Abstract

Objective: To evaluate the importance of fine needle aspiration cytology (FNAC) in the diagnosis of hepatocellular carcinoma (HCC).

Study design: We analyzed 17 cytologic and 5 architectural features in a series of 320 FNACs from HCC and compared them with 73 FNACs from benign lesions and with 705 FNACs from metastatic carcinomas. One thousand ninety-eight patients who were diagnosed by liver FNAC between December 1988 and July 1998 and had adequate follow-up were included in the study. The specimens were evaluated according to the presence or absence of the cytologic features and cellular arrangement. A stepwise logistic regression analysis was performed on the data to determine the variables predictive of HCC.

Results: Multinucleated tumor giant cells, cytoplasmic hyaline and central sinusoidal pattern were selected as the 3 most predictive parameters for differentiated reactive hepatocytes from HCC (P < .0001), while bile, centrally located nucleus in an atypical cell and intranuclear inclusion were selected as the 3 most predictive parameters for differentiated metastatic carcinoma from HCC (P < .0001-< .001) by stepwise logistic regression analysis.

Conclusion: In the 1,098 patients suspected of having hepatic malignancy, a correct diagnosis was made by a combination of the above features. The sensitivity of this procedure for hepatic malignancy was 99.5%, and the specificity was 100%.

MeSH terms

  • Adenoma, Liver Cell / pathology
  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biopsy, Needle
  • Carcinoma, Hepatocellular / pathology*
  • Cell Nucleus / pathology
  • Diagnosis, Differential
  • Female
  • Hepatitis B / pathology
  • Hepatitis C / pathology
  • Hepatocytes / pathology*
  • Humans
  • Inclusion Bodies / pathology
  • Liver / pathology*
  • Liver Neoplasms / pathology*
  • Male
  • Middle Aged
  • Retrospective Studies