Objective: To produce anti- p185erbB2 monoclonal antibody and investigate its biological activities on cancer cells that overexpress p185erbB2.
Methods: BALB/c mice were immunized with NIH3T3-erbB2 cell to prepare the anti- p185erbB2 monoclonal antibody. The specificity of the monoclonal antibody 1H3 was identified with ELISA, Western blot, immunoprecipitation and immunohistochemistry assays. The effects of monoclonal antibody 1H3 on the proliferation of cell lines that overexpressed p185erbB2 were measured by MTT assay.
Results: One strain of monoclonal antibody1H3 was obtained. In ELISA assay, 1H3 specifically reacted with cells overexpressing p185erbB2, but had no reaction with cells not overexpressing p185erbB2. The specificity of the monoclonal antibody 1H3 was verified by immunoprecipitation, Western blot and immunohistochemistry techniques. MTT assay was used to confirm that 1H3 could inhibit the growth of cells that overexpressed p185erbB2 to different extent.
Conclusion: The monoclonal antibody 1H3 obtained can specifically bind to p185erbB2 and significantly inhibit proliferation of cells overexpressing p185erbB2.