Abstract
The potential for enhancing the immunogenicity of recombinant (baculovirus-derived) influenza hemagglutinin (rHA) was investigated by comparing the immune responses elicited in mice by an intranasal (i.n.) rHA formulated with Proteosomes, with those induced by intramuscular (i.m.) or i.n. rHA alone. The Proteosome-rHA vaccine induced mucosal responses in the respiratory tract, as well as high serum IgG and hemagglutination inhibition (HAI) titers. In contrast, rHA alone given i.m. induced serum IgG without mucosal responses and was ineffective at inducing either mucosal or systemic responses when given i.n. Only mice immunized with the Proteosome-rHA vaccine were completely protected from both death and acute morbidity following live virus challenge, indicating that the i.n. Proteosome-rHA vaccine induced more complete protective immunity than the same doses of unformulated rHA given i.n. or i.m.
MeSH terms
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Adjuvants, Immunologic / pharmacology*
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Administration, Intranasal
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Animals
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Antibodies, Viral / biosynthesis
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Baculoviridae / immunology*
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Chemistry, Pharmaceutical
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Cytokines / biosynthesis
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Female
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Hemagglutinins / immunology*
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Immunity, Mucosal / immunology*
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Immunoglobulin A / biosynthesis
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Immunoglobulin G / biosynthesis
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Indicators and Reagents
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Influenza A virus / immunology
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Influenza Vaccines / administration & dosage
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Influenza Vaccines / immunology*
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Mice
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Mice, Inbred BALB C
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Orthomyxoviridae Infections / immunology
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Orthomyxoviridae Infections / prevention & control
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Phenotype
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Spleen / cytology
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Spleen / immunology
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Vaccines, DNA / administration & dosage
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Vaccines, DNA / immunology*
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Vaccines, Synthetic / immunology
Substances
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Adjuvants, Immunologic
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Antibodies, Viral
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Cytokines
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Hemagglutinins
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Immunoglobulin A
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Immunoglobulin G
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Indicators and Reagents
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Influenza Vaccines
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Vaccines, DNA
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Vaccines, Synthetic