Abstract
Objective:
To investigate the transduction of Wnt/Frizzled signaling pathway, especially the function of T cell factor 4 (TCF(4)), in renal carcinoma.
Methods:
A renal carcinoma yeast two hybrid library and a human TCF(4) yeast two hybrid expression vector were constructed. Proteins interacting with the bait protein human TCF(4) were obtained from the renal carcinoma yeast two hybrid library by reverse yeast two hybrid system.
Results:
67 positive clones interacting with the bait protein TCF(4) were obtained by reverse yeast two hybrid system, including 18 beta-catenin clones, 24 TCF(4) clones and 25 unknown clones.
Conclusion:
Wnt/Frizzled signaling pathway exists in renal carcinoma. TCF(4), its important signal factor, interacts with beta-catenin and forms homodimer or homocopolymer by itself, thus displaying its constitutive transcriptional activity.
MeSH terms
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Adenocarcinoma, Clear Cell / genetics
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Adenocarcinoma, Clear Cell / metabolism
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Cytoskeletal Proteins / metabolism
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Frizzled Receptors
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Humans
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Kidney Neoplasms / genetics*
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Kidney Neoplasms / metabolism
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Proteins / metabolism*
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Proto-Oncogene Proteins / metabolism*
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Signal Transduction* / physiology*
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TCF Transcription Factors
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Trans-Activators / biosynthesis
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Trans-Activators / genetics
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Trans-Activators / metabolism
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Transcription Factor 7-Like 2 Protein
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Transcription Factors / biosynthesis
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Two-Hybrid System Techniques
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Wnt2 Protein
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Yeasts / genetics
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beta Catenin
Substances
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CTNNB1 protein, human
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Cytoskeletal Proteins
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Frizzled Receptors
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Proteins
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Proto-Oncogene Proteins
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TCF Transcription Factors
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TCF7L2 protein, human
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Trans-Activators
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Transcription Factor 7-Like 2 Protein
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Transcription Factors
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Wnt2 Protein
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beta Catenin