Abstract
We isolated mutant YM644, which showed elevated resistance to norfloxacin, ethidium bromide, acriflavine, and rhodamine 6G, from Pseudomonas aeruginosa YM64, a strain that lacks four major multidrug efflux pumps. The genes responsible for the resistance were mexHI-opmD. Elevated ethidium extrusion was observed with cells of YM644 and YM64 harboring a plasmid carrying the genes. Disruption of the genes in the chromosomal DNA of YM644 made the cells sensitive to the drugs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / metabolism
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Anti-Bacterial Agents / pharmacology
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Cloning, Molecular
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Drug Resistance, Multiple, Bacterial / genetics
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Gene Deletion
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Membrane Transport Proteins / genetics*
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Membrane Transport Proteins / metabolism*
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Mutation / genetics*
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Plasmids / genetics
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Pseudomonas aeruginosa / genetics*
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Pseudomonas aeruginosa / metabolism*
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Tetracycline / pharmacology
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Vanadium / pharmacology
Substances
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Anti-Bacterial Agents
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Membrane Transport Proteins
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OpmD protein, Pseudomonas aeruginosa
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Vanadium
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Tetracycline