Production and characterization of two monoclonal antibodies to human glutathione peroxidase

Hybridoma. 1992 Dec;11(6):795-801. doi: 10.1089/hyb.1992.11.795.

Abstract

Glutathione peroxidase (GSH-Px) is an important selenium-containing enzyme which protects cells from oxidative damage. Two hybridoma clones (GPX-121 and GPX-347), producing mouse IgG1 monoclonal antibodies specific for GSH-Px, were established. Immunoblot analysis revealed that GPX-347 was specific for human GSH-Px, while GPX-121 cross-reacted with human, rat, mouse and rabbit GSH-Px. Correlation between GSH-Px content and its enzymatic activity was investigated in erythrocytes of 76 humans and in human lung adenocarcinoma PC-9 cells by using a sandwich type ELISA. The results indicated that GSH-Px activity was expressed higher than expected from GSH-Px content especially in the range of low GSH-Px concentration. PC-9 cells selenium depleted medium did not stain but the cytoplasm of PC-9 cells grown in medium supplemented with selenium stained strongly.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / enzymology
  • Adult
  • Aged
  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Monoclonal / isolation & purification
  • Cross Reactions
  • Culture Media
  • Enzyme-Linked Immunosorbent Assay
  • Erythrocytes / enzymology
  • Female
  • Glutathione Peroxidase / blood
  • Glutathione Peroxidase / immunology*
  • Humans
  • Hybridomas / immunology
  • Immunoglobulin G / immunology*
  • Lung Neoplasms / enzymology
  • Male
  • Mammals / immunology
  • Mice
  • Mice, Inbred BALB C / immunology
  • Middle Aged
  • Neoplasm Proteins / immunology
  • Selenium / pharmacology
  • Species Specificity
  • Tumor Cells, Cultured / drug effects

Substances

  • Antibodies, Monoclonal
  • Culture Media
  • Immunoglobulin G
  • Neoplasm Proteins
  • Glutathione Peroxidase
  • Selenium