Objective: Considering that the complement C3d plays a stronger positive role in the control of immunologic system, we constructed the recombinant plasmid of the molecular-adjuvant gene of the complement C3d in order to enhance the immunologic efficacy of the gene vaccine.
Methods: The main points were amplifying the segment of complement C3d gene by using of PCR; inserting it into PGEM-T; detecting it by digesting with Eco RI and sequencing; the whole length being 897 bp; then sub-cloning it into pVAX1.
Results: The DNA sequencing confirmed the C3d sequence.
Conclusion: We have successfully cloned the gene of C3d and constructed the recombinant plasmid of pVAX1 C3d.