HMG-CoA reductase inhibitor cerivastatin inhibits interleukin-6 expression and secretion in human adipocytes

Horm Metab Res. 2003 Aug;35(8):466-70. doi: 10.1055/s-2003-41803.

Abstract

Human adipose tissue is a main contributor to plasma levels of pro-inflammatory cytokine IL-6. How IL-6 expression is regulated in adipocytes remains unclear. In the current study, we investigated the effect of the HMG-CoA reductase inhibitor, cerivastatin, on the production of IL-6 from cultured human adipocytes. Cerivastatin reduced both IL-6 mRNA and secretion in a dose- and time-dependent manner. The inhibitory effect on IL-6 mRNA was prevented by the intermediates of the cholesterol synthesis pathway, mevalonate and geranyl-geranyl-phyrophosphate (GGPP) but not by farnesyl-pyrophosphate. This suggests the involvement of geranylgeranyl-modified intermediates in the effect of cerivastatin on IL-6. Moreover, cerivastatin induced an inactivation of the phosphorylation of the p65 subunit of NFkappaB which was prevented by GGPP. Our data suggest that cerivastatin exerts an anti-inflammatory effect by down-regulating IL-6 levels in adipocytes, which seems to be mediated by reduced production of GGPP and interference with the NFkappaB pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / drug effects*
  • Adipocytes / metabolism*
  • Adult
  • Cells, Cultured
  • Female
  • Humans
  • Hydroxymethylglutaryl-CoA Reductase Inhibitors / pharmacology*
  • Interleukin-6 / antagonists & inhibitors*
  • Interleukin-6 / genetics
  • Mevalonic Acid / pharmacology
  • Middle Aged
  • NF-kappa B / metabolism
  • Phosphorylation / drug effects
  • Pyridines / pharmacology*
  • RNA, Messenger / metabolism
  • Transcription Factor RelA

Substances

  • Hydroxymethylglutaryl-CoA Reductase Inhibitors
  • Interleukin-6
  • NF-kappa B
  • Pyridines
  • RNA, Messenger
  • Transcription Factor RelA
  • cerivastatin
  • Mevalonic Acid