Highly sensitive PCR assay for routine diagnosis of African swine fever virus in clinical samples

J Clin Microbiol. 2003 Sep;41(9):4431-4. doi: 10.1128/JCM.41.9.4431-4434.2003.

Abstract

This work provides a novel, highly sensitive, hot start PCR method for rapid and specific detection of African swine fever virus (ASFV) that can be used as a routine diagnostic test for ASFV in surveillance, control, and eradication programs. A confirmatory test of the specificity of this method based on restriction endonuclease analysis was also developed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • African Swine Fever Virus / isolation & purification*
  • DNA, Viral / analysis
  • Humans
  • Polymerase Chain Reaction / methods*
  • Retrospective Studies
  • Sensitivity and Specificity

Substances

  • DNA, Viral