Abstract
A cDNA encoding a bHLH transcription factor was isolated by the yeast one-hybrid system from a Catharanthus roseus cDNA library using the G-box element of the Strictosidine synthase gene promoter as bait. The corresponding protein (named CrMYC1) was shown to bind specifically to the G-box in yeast. In C. roseus suspension cells CrMYC1 mRNA levels are induced by fungal elicitor and jasmonate suggesting that CrMYC1 may be involved in the regulation of gene expression in response to these signals.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Asteraceae / drug effects
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Asteraceae / genetics
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Asteraceae / metabolism*
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Carbon-Nitrogen Lyases / genetics*
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Cyclopentanes / pharmacology*
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DNA-Binding Proteins / metabolism
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Gene Expression Regulation, Plant / drug effects
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Gene Expression Regulation, Plant / genetics*
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Helix-Loop-Helix Motifs
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Molecular Sequence Data
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Oxylipins
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Plant Growth Regulators / pharmacology
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Promoter Regions, Genetic*
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Substrate Specificity
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Transcription Factors / genetics*
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Transcription Factors / metabolism
Substances
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Cyclopentanes
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DNA-Binding Proteins
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Oxylipins
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Plant Growth Regulators
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Plant Proteins
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Transcription Factors
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jasmonic acid
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Carbon-Nitrogen Lyases
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strictosidine synthetase