Abstract
A gram-negative, alkalotolerant bacterium, isolated from the soil continually drained with industrial wastewater and identified as gamma-proteobacterium by partial 16S rRNA sequence analysis, produced a polyphenol oxidase, which showed laccase but not tyrosinase activity. The organism grew well from pH 6 to 10 and produced laccase maximally at pH 10. The enzyme was stable from pH 3 to 10.6 for at least 24 h and was optimally active at 55 degrees C and pH 6.5 in a 5 min assay.
MeSH terms
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DNA, Bacterial / genetics
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Gammaproteobacteria / enzymology*
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Gammaproteobacteria / growth & development
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Gammaproteobacteria / isolation & purification
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Hydrogen-Ion Concentration
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Industrial Waste
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Laccase / biosynthesis*
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Laccase / metabolism
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Melanins / metabolism
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Molecular Sequence Data
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Polymerase Chain Reaction
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RNA, Ribosomal, 16S / analysis
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Soil / analysis*
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Soil Microbiology*
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Soil Pollutants
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Substrate Specificity
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Temperature
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Tyrosine / metabolism
Substances
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DNA, Bacterial
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Industrial Waste
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Melanins
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RNA, Ribosomal, 16S
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Soil
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Soil Pollutants
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Tyrosine
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Laccase