Abstract
In the enzymatic synthesis of cefaclor, 3-chloro-7-d-(2-phenylglycinamide)-3-cephem-4-carboxylic acid, from phenylglycine methyl ester and 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid, the in situ product could influence both the overall conversion and hydrolysis of the ester. Optimization of the parameters, such as pH 6.2, 5 degrees C and substrate molar ratio of 2:1, made in situ product removal improve the overall conversion from 64% to 85% (mol/mol).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bioreactors
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Cefaclor / chemical synthesis*
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Cefaclor / chemistry
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Cephalosporins / metabolism*
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Chromatography, High Pressure Liquid
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Enzymes, Immobilized / metabolism*
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Glycine / analogs & derivatives*
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Glycine / metabolism*
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Hydrogen-Ion Concentration
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Hydrolysis
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Models, Chemical
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Penicillin Amidase / metabolism*
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Spectrophotometry
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Substrate Specificity
Substances
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Cephalosporins
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Enzymes, Immobilized
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methyl phenylglycine
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Cefaclor
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7-aminodesacetoxycephalosporanic acid
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Penicillin Amidase
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Glycine