Human leukocyte antigen-G (HLA-G) expression in biliary epithelial cells is associated with allograft acceptance in liver-kidney transplantation

J Hepatol. 2003 Oct;39(4):587-94. doi: 10.1016/s0168-8278(03)00354-4.

Abstract

Background/aims: Liver allograft is known to protect simultaneously transplanted organs from acute rejection. We have reported that only 6% of combined liver-kidney recipients, versus 32.5% of kidney recipients, develop kidney graft acute rejection. Release of soluble human leukocyte antigen (HLA) molecules by the liver has been proposed as a possible tolerogenic mechanism involved in the better acceptance of double transplants. The HLA-G molecule is acknowledged to possess tolerogenic properties.

Methods: We investigated the involvement of HLA-G in allogeneic transplant acceptance by analyzing its expression in kidney and liver biopsies of 40 combined transplanted patients.

Results: We demonstrate the presence of HLA-G in 14 out of 40 liver and five out of nine kidney transplants biopsies. HLA-G is expressed de novo by cells that are otherwise frequently susceptible target cells of acute rejection, i.e. liver biliary and renal tubular epithelial cells. We show a significant association between HLA-G expression in liver biliary epithelial cells and the absence of liver graft rejection. No acute or chronic rejection of the kidney graft was observed in patients in whom HLA-G was expressed in the liver graft.

Conclusions: HLA-G expression in the liver allograft is associated with a lower frequency of hepatic and renal acute rejection and may be involved in the acceptance of simultaneously transplanted organs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Biliary Tract / cytology
  • Biliary Tract / metabolism*
  • Biopsy
  • Epithelial Cells / metabolism
  • Female
  • Graft Rejection / prevention & control
  • Graft Survival*
  • HLA Antigens / metabolism*
  • HLA-G Antigens
  • Histocompatibility Antigens Class I / metabolism*
  • Humans
  • Immune Tolerance*
  • Immunohistochemistry
  • Kidney / metabolism
  • Kidney / pathology
  • Kidney Transplantation / immunology*
  • Liver / metabolism
  • Liver / pathology
  • Liver Transplantation / immunology*
  • Male
  • Middle Aged
  • Transplantation, Homologous

Substances

  • HLA Antigens
  • HLA-G Antigens
  • Histocompatibility Antigens Class I