From February 1999 to February 2000, sand flies were captured weekly with CDC light traps at five sites in the urban area of Campo Grande, Mato Grosso do Sul State, Brazil. Traps were placed in 11 different ecotopes in the environment (ground level, tree canopies, and forest edge) and the peridomicile (chicken coops and banana trees). A total of 1,245 sand flies were captured, belonging to 28 species: 4 species from genus Brumptomyia Fran a & Parrot, 1921 and 24 from genus Lutzomyia Fran a, 1924. The species were: B. avellari, B. brumpti, B. galindoi, B. pintoi, L. aragaoi, L. bourrouli, L. campograndensis, L. cerradincola, L. christenseni, L. claustrei, L. cortelezzii, L. corumbaensis, L. cruzi, L. damascenoi, L. flaviscutellata, L. hermanlenti, L. lenti, L. longipalpis, L. longipennis, L. migonei, L. punctigeniculata, L. quinquefer, L. renei, L. shannoni, L. sordellii, L. teratodes, L. termitophila, and L. whitmani. L. longipalpis and L. cruzi, vectors of visceral leishmaniasis, and L. whitmani, L. flaviscutellata and L. migonei, vectors of cutaneous leishmaniasis, were captured in the urban area. The most frequent species were L. termitophila, L. aragaoi, L. lenti, L. longipennis, and L. longipalpis.