Using gel chromatography of Sephadex G-75 superfine connected with Sephadex G-50 fine column, three human gamma-crystallins (gamma 1, gamma 2, gamma 3) could be obtained. Seven agglutinins (LCA, SBA, DBA, PNA, BSL, RCA and UEA) were used to detect the sugar of sub-gamma-crystallins, which had been transferred to nitrocellulose membrane and finally stained with ABC reagents and the substrate of HPR. These results suggested that gamma 2- and gamma 3-crystallins contain sugar, but gamma 1-crystallin has no sugar. There is a decrease of carbohydrate of gamma 2 and gamma 3 as the development of the lens (esp. the former). It was postulated that in gamma-crystallins (gamma 2, gamma 3) the sugar components helped stabilizing the molecular structure (folding polypeptide, prevent protein breaking). The sugar components decreased with age by the post-translation modification.