Abstract
Brefeldin A (BFA), a fungal metabolite that blocks transport of newly synthesized proteins from the endoplasmic reticulum, was found to inhibit poliovirus replication 10(5)- to 10(6)-fold. BFA does not inhibit entry of poliovirus into the cell or translation of viral RNA. Poliovirus RNA synthesis, however, is completely inhibited by BFA. A specific class of membranous vesicles, with which the poliovirus replication complex is physically associated, is known to proliferate in poliovirus-infected cells. BFA may inhibit poliovirus replication by preventing the formation of these vesicles.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviridae / drug effects
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Adenoviridae / genetics
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Adenoviridae / physiology
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Antiviral Agents / pharmacology*
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Brefeldin A
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Cyclopentanes / pharmacology*
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Fluorescent Antibody Technique
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HeLa Cells
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Humans
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Kinetics
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Lipids / biosynthesis
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Poliovirus / drug effects*
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Poliovirus / genetics
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Poliovirus / metabolism
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Poliovirus / physiology
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Protein Biosynthesis
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RNA, Viral / biosynthesis*
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Virus Replication / drug effects*
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Virus Replication / genetics
Substances
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Antiviral Agents
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Cyclopentanes
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Lipids
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RNA, Viral
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Brefeldin A