Abstract
We describe the cloning of a multifunctional folic acid synthesis (fas) gene from Pneumocystis carinii. The nucleotide sequence contains an open reading frame interrupted by three introns, that encodes a protein of 740 amino acids with an Mr of 97,278. The predicted Fas protein has homology to two enzyme domains, dihydropteroate synthase and 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, both of which are involved in folate synthesis, and at least one other region of unknown function.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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Dihydropteroate Synthase / genetics*
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Diphosphotransferases*
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Folic Acid / biosynthesis*
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Genes, Fungal / genetics*
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Molecular Sequence Data
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Phosphotransferases / genetics*
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Pneumocystis / enzymology*
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Pneumocystis / genetics
Substances
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Folic Acid
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Dihydropteroate Synthase
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Phosphotransferases
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Diphosphotransferases
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2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
Associated data
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GENBANK/M62416
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GENBANK/M62417
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GENBANK/M63477
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GENBANK/M63478
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GENBANK/M63479
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GENBANK/M86602
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GENBANK/S40001
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GENBANK/S40008
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GENBANK/S40009
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GENBANK/S40010