Abstract
The native structure of the calcium release channel (ryanodine receptor) from rabbit skeletal muscle has been analyzed in two dimensions from electron micrographs of frozen hydrated specimens. Within a resolution of 3.0 nm there is excellent agreement between the structure as seen in vitreous water and in negative stained specimens. Features seen in the three-dimensional reconstruction of the negatively stained channel can be identified in the projection of the unstained receptor.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Biophysical Phenomena
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Biophysics
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Calcium Channels / ultrastructure*
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Freezing
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Microscopy, Electron
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Muscle Proteins / ultrastructure*
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Protein Conformation
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Rabbits
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Receptors, Cholinergic / ultrastructure*
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Ryanodine Receptor Calcium Release Channel
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Sarcoplasmic Reticulum / ultrastructure
Substances
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Calcium Channels
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Muscle Proteins
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Receptors, Cholinergic
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Ryanodine Receptor Calcium Release Channel