Solid-phase synthesis of an RNA nucleopeptide fragment from the nucleoprotein of poliovirus

C M Dreef-Tromp; H van den Elst; J E van den Boogaart; G A van der Marel; J H van Boom

doi:10.1093/nar/20.10.2435

Solid-phase synthesis of an RNA nucleopeptide fragment from the nucleoprotein of poliovirus

Nucleic Acids Res. 1992 May 25;20(10):2435-9. doi: 10.1093/nar/20.10.2435.

Authors

C M Dreef-Tromp¹, H van den Elst, J E van den Boogaart, G A van der Marel, J H van Boom

Affiliation

¹ Gorlaeus Laboratory, Leiden, The Netherlands.

Abstract

The naturally occurring RNA-nucleopeptide H-Ala-Tyr[5'-pUUAAAAC-3']-NH2 is prepared via a solid-phase phosphite triester approach using N-SiOMB/O-TBDMS-protected nucleosides. Preliminary 1H-NMR studies show that the peptidyl unit has a remarkable effect on the conformational behaviour of the RNA moiety in the nucleopeptide.

MeSH terms

Magnetic Resonance Spectroscopy
Nucleic Acid Conformation
Nucleoproteins / chemistry
Oligoribonucleotides / chemistry*
Peptide Fragments / chemical synthesis*
Peptide Fragments / chemistry
Poliovirus / chemistry*
RNA, Viral / chemistry*
Ribonucleoproteins / chemical synthesis*
Ribonucleoproteins / chemistry
Viral Proteins / chemical synthesis*
Viral Proteins / chemistry

Substances

Nucleoproteins
Oligoribonucleotides
Peptide Fragments
RNA, Viral
Ribonucleoproteins
Viral Proteins