Pilocarpine-induced increases in the activity of 6-phosphofructo-2-kinase and the fructose-2,6-bisphosphate content of rat salivary glands

Arch Oral Biol. 1992;37(6):483-7. doi: 10.1016/0003-9969(92)90104-g.

Abstract

The activity of 6-phosphofructo-1-kinase (PFK-1), an important regulatory enzyme of glycolysis, was determined after injection of the sialagogue pilocarpine. The fructose-2,6-bisphosphate content of the glands and 6-phosphofructo-2-kinase (PFK-2) activity were also measured. The increase in PFK-1 activity after pilocarpine treatment was likely to be due to the increase in the content of its potent modulator, fructose-2,6-bisphosphate. This in turn was assumed to be due to the increase in the activity of the active form of PFK-2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Fructosediphosphates / analysis*
  • Glycogen / analysis
  • Male
  • Parotid Gland / chemistry*
  • Parotid Gland / drug effects*
  • Parotid Gland / enzymology
  • Phosphofructokinase-1 / metabolism*
  • Phosphofructokinase-2
  • Phosphotransferases / metabolism*
  • Pilocarpine / pharmacology*
  • Rats
  • Rats, Inbred Strains
  • Submandibular Gland / chemistry*
  • Submandibular Gland / drug effects*
  • Submandibular Gland / enzymology
  • Time Factors

Substances

  • Fructosediphosphates
  • Pilocarpine
  • fructose 2,6-diphosphate
  • Glycogen
  • Phosphotransferases
  • Phosphofructokinase-2
  • Phosphofructokinase-1