Abstract
The Xanthomonas campestris catabolite gene activator protein-like protein (CLP) can substitute for the Escherichia coli catabolite gene activator protein (CAP) in transcription activation at the lac promoter (V. de Crecy-Lagard, P. Glaser, P. Lejeune, O. Sismeiro, C. Barber, M. Daniels, and A. Danchin, J. Bacteriol. 172:5877-5883, 1990). We show that CLP has the same DNA binding specificity as CAP at positions 5, 6, and 7 of the DNA half site. In addition, we show that the amino acids at positions 1 and 2 of the recognition helix of CLP are identical to the amino acids at positions 1 and 2 of the recognition helix of CAP:i.e., Arg at position 1 and Glu at position 2.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins*
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Base Sequence
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Cyclic AMP Receptor Protein / chemistry
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Cyclic AMP Receptor Protein / genetics
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Cyclic AMP Receptor Protein / metabolism*
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DNA, Bacterial / metabolism*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Molecular Sequence Data
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Sequence Homology, Nucleic Acid
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Substrate Specificity
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Transcription Factors*
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Transcription, Genetic
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Xanthomonas / metabolism*
Substances
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Bacterial Proteins
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Cyclic AMP Receptor Protein
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DNA, Bacterial
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DNA-Binding Proteins
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Transcription Factors
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CAP-like protein, Xanthomonas campestris
Associated data
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GENBANK/M90093
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GENBANK/M92289
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GENBANK/M94059
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GENBANK/M94060
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GENBANK/M94061
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GENBANK/M94062
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GENBANK/X64378
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GENBANK/X64379
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GENBANK/X64380
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GENBANK/X64381