Abstract
Interferon regulatory factor-1 (IRF-1) gene expression is rapidly upregulated in the prolactin (PRL)-activated Nb2 rat T lymphoma cell line. To further elucidate its role as a T cell activation molecule, IRF-1 gene expression in response to various T cell stimuli was examined. In Nb2 T cells, PRL induced two peaks of IRF-1 gene expression: a rapid, transient peak at 1 h and a sustained peak at 12 h. PRL subsequently induced interferon-gamma (IFN-gamma) gene expression at 3-6 h. However, the early induction of IRF-1 and IFN-gamma does not appear to be interdependent. Interleukin-2 (IL-2) also induced IRF-1 gene expression in Nb2 T cells but only one broad peak at 10 h was observed. In primary mouse splenocytes, concanavalin A induced rapid and transient expression of the IRF-1 gene; maximal expression occurred by 6 h, and then returned to basal levels by 12-15 h. These results provide additional evidence for the importance of IRF-1 in T cell activation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cells, Cultured
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Concanavalin A / pharmacology*
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DNA-Binding Proteins / biosynthesis*
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Gene Expression Regulation, Neoplastic / drug effects
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Interferon Regulatory Factor-1
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Interleukin-2 / pharmacology*
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Lymphoma, T-Cell / pathology
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Mice
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Mice, Inbred BALB C
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Neoplastic Stem Cells / drug effects
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Neoplastic Stem Cells / metabolism
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Phosphoproteins / biosynthesis*
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Prolactin / pharmacology*
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RNA, Messenger / biosynthesis*
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Rats
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Receptors, Cell Surface / drug effects
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Recombinant Proteins / pharmacology
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Signal Transduction / drug effects
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Stimulation, Chemical
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T-Lymphocytes / drug effects
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T-Lymphocytes / metabolism*
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / metabolism
Substances
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DNA-Binding Proteins
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Interferon Regulatory Factor-1
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Interleukin-2
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Irf1 protein, mouse
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Irf1 protein, rat
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Phosphoproteins
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RNA, Messenger
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Receptors, Cell Surface
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Recombinant Proteins
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Concanavalin A
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Prolactin