Inosine-containing primers in human papillomavirus detection by polymerase chain reaction

G Novelli; M Gennarelli; L De Santis; P Angeloni; B Dallapiccola

doi:10.1016/0753-3322(92)90025-3

Inosine-containing primers in human papillomavirus detection by polymerase chain reaction

Biomed Pharmacother. 1992;46(4):167-9. doi: 10.1016/0753-3322(92)90025-3.

Authors

G Novelli¹, M Gennarelli, L De Santis, P Angeloni, B Dallapiccola

Affiliation

¹ Department of Public Health and Cell Biology, 2nd University of Rome, Italy.

PMID: 1330051
DOI: 10.1016/0753-3322(92)90025-3

Abstract

A PCR protocol has been developed using inosine-containing primers for human papilloma virus (HPV) detection. The HPV16 L1 region from CaSki-positive cells was efficiently amplified by a single reaction and directly analyzed by agarose gel electrophoresis. The method was found to be sensitive and reproducible, and it easy to use for HPV typing and screening.

MeSH terms

Base Sequence
Humans
In Vitro Techniques
Inosine / chemistry*
Papillomaviridae / isolation & purification*
Polymerase Chain Reaction / methods*

Substances

Inosine