Angiotensin II induces formation of the early growth response gene-1 protein in rat vascular smooth muscle cells

A Sachinidis; P Weisser; Y Ko; K Schulte; K Meyer zu Brickwedde; L Neyses; H Vetter

doi:10.1016/0014-5793(92)81423-j

Angiotensin II induces formation of the early growth response gene-1 protein in rat vascular smooth muscle cells

FEBS Lett. 1992 Nov 23;313(2):109-12. doi: 10.1016/0014-5793(92)81423-j.

Authors

A Sachinidis¹, P Weisser, Y Ko, K Schulte, K Meyer zu Brickwedde, L Neyses, H Vetter

Affiliation

¹ Medizinische Universitäts-Poliklinik, Bonn, Germany.

PMID: 1330700
DOI: 10.1016/0014-5793(92)81423-j

Abstract

The effect of angiotensin II (Ang II) on the early growth response gene-1 (Egr-1) mRNA, on the Egr-1 protein and on the phosphoinositide PI turnover signalling system was investigated in the presence and absence of EXP3174, a potent non-peptide Ang II receptor antagonist. Ang II induced an accumulation of 3.4 kb Egr-1 mRNA and the 80 kDa Egr-1 protein, with a maximum at 30 min and 60 min, respectively. EXP3174 blocked the Ang II-induced increase of inositol phosphates, Egr-1 mRNA and the Egr-1 protein, suggesting the involvement of the PI signalling system by the expression of the Egr-1 gene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Angiotensin II / pharmacology*
Animals
Cells, Cultured
DNA-Binding Proteins / biosynthesis*
DNA-Binding Proteins / genetics
Early Growth Response Protein 1
Female
Immediate-Early Proteins*
Muscle, Smooth, Vascular / drug effects
Muscle, Smooth, Vascular / metabolism*
Phosphatidylinositols / metabolism
RNA, Messenger / analysis
Rats
Rats, Inbred WKY
Transcription Factors / biosynthesis*
Transcription Factors / genetics

Substances

DNA-Binding Proteins
Early Growth Response Protein 1
Egr1 protein, rat
Immediate-Early Proteins
Phosphatidylinositols
RNA, Messenger
Transcription Factors
Angiotensin II