Isolation of amine oxidase from bovine plasma by a two-step procedure

F Vianello; M L Di Paolo; L Zennaro; R Stevanato; A Rigo

doi:10.1016/s1046-5928(05)80036-1

Isolation of amine oxidase from bovine plasma by a two-step procedure

Protein Expr Purif. 1992 Oct;3(5):362-7. doi: 10.1016/s1046-5928(05)80036-1.

Authors

F Vianello¹, M L Di Paolo, L Zennaro, R Stevanato, A Rigo

Affiliation

¹ Department of Biological Chemistry, University of Padova, Italy.

PMID: 1333859
DOI: 10.1016/s1046-5928(05)80036-1

Abstract

A novel method for isolation of amine oxidase from bovine plasma is reported; it involves a two-step procedure, namely ammonium sulfate fractionation and affinity chromatography with elution by aniline, which is a competitive inhibitor of the enzyme. A homogeneous enzyme, characterized by a specific activity of 0.44 U/mg, was obtained with a yield higher than 50%.

MeSH terms

Amine Oxidase (Copper-Containing)*
Amines
Animals
Cattle
Chromatography, Affinity
Electron Spin Resonance Spectroscopy
Electrophoresis, Polyacrylamide Gel
Oxidoreductases Acting on CH-NH Group Donors / antagonists & inhibitors
Oxidoreductases Acting on CH-NH Group Donors / blood*
Oxidoreductases Acting on CH-NH Group Donors / isolation & purification*
Sepharose / analogs & derivatives

Substances

Amines
aminohexyl-sepharose
Sepharose
Amine Oxidase (Copper-Containing)
Oxidoreductases Acting on CH-NH Group Donors